P-193: The Association of Apolipoprotien E Polymorphisms with Recurrent Pregnancy Loss

Background: The role of apolipoprotien E polymorphisms do not diagnostic correctly in recurrent pregnancy loss etiology but Apo E has been shown to play an important role in lipid metabolism in pregnancy. We evaluated these polymorphisms in Iranian women with unexplained recurrent pregnancy loss. Materials and Methods: 5 ml Blood were sampling from 81 women with a history of two or more consecutive spontaneous abortions and 81 women with at least two live births and not miscarriage. DNA was extracted from the leukocytes and PCR amplification of Apo E was performed. For determining Apo E genotypes used restriction fragment length polymorphism analysis. Results: Women with consecutive spontaneous abortions had greater significantly frequency from Apo E E3/ E4 and E4/E4 genotype (19.75%) comparing with control group (2.46%) (p=0.032). Conclusion: Polymorphism Apo E4 may help to thrombophilic risk factors in loss of fetus during pungency. Our data supported association polymorphisms apolipoprotein E with recurrent pregnancy loss.